RESUMO
In a previous study, three bacterial strains isolated from tropical hydrocarbon-contaminated soils and phylogenetically identified as Achromobacter sp. strain SL1, Pseudomonas sp. strain SL4 and Microbacterium esteraromaticum strain SL6 displayed angular dioxygenation and mineralization of carbazole in batch cultures. In this study, the ability of these isolates to survive and enhance carbazole degradation in soil were tested in field-moist microcosms. Strain SL4 had the highest survival rate (1.8 x 107 cfu/g) after 30 days of incubation in sterilized soil, while there was a decrease in population density in native (unsterilized) soil when compared with the initial population. Gas chromatographic analysis after 30 days of incubation showed that in sterilized soil amended with carbazole (100 mg/kg), 66.96, 82.15 and 68.54% were degraded by strains SL1, SL4 and SL6, respectively, with rates of degradation of 0.093, 0.114 and 0.095 mg kg−1 h−1. The combination of the three isolates as inoculum in sterilized soil degraded 87.13% carbazole at a rate of 0.121 mg kg−1 h−1. In native soil amended with carbazole (100 mg/kg), 91.64, 87.29 and 89.13% were degraded by strains SL1, SL4 and SL6 after 30 days of incubation, with rates of degradation of 0.127, 0.121 and 0.124 mg kg−1 h−1, respectively. This study successfully established the survivability (> 106 cfu/g detected after 30 days) and carbazole-degrading ability of these bacterial strains in soil, and highlights the potential of these isolates as seed for the bioremediation of carbazole-impacted environments.
Assuntos
Achromobacter/química , Achromobacter/genética , Achromobacter/isolamento & purificação , Achromobacter/metabolismo , Actinobacteria/química , Actinobacteria/genética , Actinobacteria/isolamento & purificação , Actinobacteria/metabolismo , Biodegradação Ambiental/química , Biodegradação Ambiental/genética , Biodegradação Ambiental/isolamento & purificação , Biodegradação Ambiental/metabolismo , Carbazóis/química , Carbazóis/genética , Carbazóis/isolamento & purificação , Carbazóis/metabolismo , Filogenia/química , Filogenia/genética , Filogenia/isolamento & purificação , Filogenia/metabolismo , Pseudomonas/química , Pseudomonas/genética , Pseudomonas/isolamento & purificação , Pseudomonas/metabolismo , Microbiologia do Solo/química , Microbiologia do Solo/genética , Microbiologia do Solo/isolamento & purificação , Microbiologia do Solo/metabolismo , Poluentes do Solo/química , Poluentes do Solo/genética , Poluentes do Solo/isolamento & purificação , Poluentes do Solo/metabolismoRESUMO
Abstract Carbaryl is an important and widely used insecticide that pollutes soil and water systems. Bacteria from the local soil ecosystem of the Gaza Strip capable of utilizing carbaryl as the sole source of carbon and nitrogen were isolated and identified as belonging to Bacillus, Morganella, Pseudomonas, Aeromonas and Corynebacterium genera. Carbaryl biodegradation by Bacillus, Morganella and Corynebacterium isolates was analyzed in minimal liquid media supplemented with carbaryl as the only source of carbon and nitrogen. Bacillus and Morganella exhibited 94.6% and 87.3% carbaryl degradation, respectively, while Corynebacterium showed only moderate carbaryl degradation at 48.8%. These results indicate that bacterial isolates from a local soil ecosystem in the Gaza Strip are able to degrade carbaryl and can be used to decrease the risk of environmental contamination by this insecticide.
